Dunstaffnage Marine Laboratory: projects/activities

-Development of methods to enhance the rate of toxin depuration ( detoxification), especially in shellfish species of high economic value and prolonged retention e.g., King Scallops -Understanding the reaction products and metabolic transformations of toxins in shellfish tissues. -Determine the relationship between algal population dynamics ( including free cell and encysted stages ) to seasonal and spatial patterns of toxicity in shellfish populations. -Assess the effects of harmful algae on the various stages in the life history of shellfish ( Larvae, Spat, Adults ). -Investigate sampling frequencies and protocols ( live shellfish sampling ).

Although the most visible effect of fish cage aquaculture is the output of particulate organic waste, 80% of the total nutrient losses from fish farming are plant-available as potentially eutrophicating substances. This project will assess the ability of commercially important seaweeds, cultivated in the immediate vicinity of caged fish, to reduce the impact of such nutrient releases. The algae cultivated in high nutrient sites will be tested as a food source for humans and for cultivated shellfish, and a model of the distribution of dissolved contaminants from sea-cage fish farms will be developed to predict the impact of introducing algal cultivation at any site.

(a) To assemble and further develop an integrative methodology for in situ evaluation of the effects of turbidity and hypoxia on fish physiological and/or behavioural performance. (b) To determine experimentally the threshold values beyond which oxygen and turbidity levels are liable to alter fish physiological and/or behavioural performance. (c) To integrate the results obtained in a conceptual and predictive model. Main expected achievements: [1] establishment of a link between laboratory studies, studies in mesocosms and field studies, using the most advanced techniques for monitoring behaviour in various environmental conditions. [2] an understanding of the impact of water turbidity and oxygenation on three major components of the behavioural repertoire of fish: habitat selection, predator-prey interactions and schooling-aggregation. [3] Predictive ability for the effect of the environmental variables studied on ecologically relevant behaviour.

1. The improvement of the scientific basis for the management of fisheries taking elasmobranch species by: a)Species Identification /biological sampling b)Stock discrimination / separation c)Data compliation and exchange d)Data preparation, stock assessment & species vulnerability

1. To quantify the effectiveness of the biofilters in reducing the impacts of mariculture across Europe from both an economic and environmental perspective. 2. To determine the best design and placements of the biofilters, accounting for differences in geography, hydrology, nutrient input etc. between countries. 3. To examine the environmental and regulatory options governing the use of the biofilters at the end of their life-span and to provide detailed economic analyses of biofilter use compared to existing filtration methods.

1. To establish an environmental monitoring regieme during and following the period of reef complex construction using, where possible, the same static monitoring sites and transects established during the pre-deployment research, in addition to new stations 2. To develop and test models that will predict ecosystem changes caused by artifical habitat manipulation. The main model will examine whole ecosystem changes. Other models will examine hydrological profile alterations, habitat fractal dimensions and socio-economic cost benefit analysis.

1. To descirbe and compare the phylogenetic diversity and distribution of the total bacterial flora associated with G catenatum cysts and vegetative cells. 2. To culture and identify bacteria from G catenatum, and identify/characterise any bacteria capable of autonomous PST production in G. catenatum 3. To examine the effect of cyst surface sterilisation and re-introduction of bacteria on PST production in G catenatum 4. Survey bacteria for quorum sensing capability (cell signaling) and detect in situ quorum sesing in xenic G. catenatum cultures, relating to toxicity development. 5. Develop molecular markers of cross species quorum sensing, facilitating analysis of quorum sensing in uncultivated bacteria.

1. Analysis of existing data from the current shellfish monitoring programmes in order to design a suitable sampling strategy 2. Ideentification of toxic algal species in UK waters 3. Construction of a detailed time-series at several key sites in the UK for toxic phytoplankton and shellfish toxin occurence 4. Comparison of the genotype versus toxicity of suspected toxic species between sites

Objective 1: To map the structural and genetic variability, the framework-constructing potential, and the longevity of Deep Water Coral (DWC) ecosystems Objective 2: To assess hydrographic and other local physical forcing factors affecting Benthic Boundary Layer (BBL) sediment particle dynamics and POC supply in the vicinity of DWC ecosystems Objective 3: To describe the DWC ecosystem, its dynamics and functioning; investigate coral biology and behaviour and assess coral sensitivity to natural and anthropogenic stressors Objective 4: To assign a sensitivity code, identify the major conservation issues (and increase public awareness), and make recommendations for the sustainable use of the DWC ecosystem

1. To determine the effects of each of several sealice treatment chemicals on macrofaunal assemblages 2. To determine the effects of each of several sealice treatment chemicals on zooplankton assemblages 3. To determine the effects of each of several sealice treatment chemicals on meiofaunal assemblages 4. To determine the effects of each of several sealice treatment chemicals on benthic diatom assemblages 5. To determine the effects of each of several sealice treatment chemicals on phytoplankton assemblages 6. To determine the effects of each of several sealice treatment chemicals on macroalgal and littoral assemblages 7. To measure the concentrations of each of several sea lice treatment chemicals in the environment post-treatment 8. To determine the significant correlations between ecosystem responses, time and therapeutant concentration to determine the proportion of the observed environmental variance attributal to the treatments against a background of responses due to other parameters such as waste organic materials and nutrients 9. To model the dispersion and or depostion of farm wastes including of each of several sea lice treatment chemicals in the marine environment post treatment and to incorporate terms relating to the toxicity of these chemicals to certain parts of the ecosystem (e.g. the macrofauna)

1. To develop a system of photoactive biocides for treating sea lice and biofouling (Further details in confidence)