Dunstaffnage Marine Laboratory: projects/activities

-Development of methods to enhance the rate of toxin depuration ( detoxification), especially in shellfish species of high economic value and prolonged retention e.g., King Scallops -Understanding the reaction products and metabolic transformations of toxins in shellfish tissues. -Determine the relationship between algal population dynamics ( including free cell and encysted stages ) to seasonal and spatial patterns of toxicity in shellfish populations. -Assess the effects of harmful algae on the various stages in the life history of shellfish ( Larvae, Spat, Adults ). -Investigate sampling frequencies and protocols ( live shellfish sampling ).

-Quantify changes in ice dynamics and characteristics resulting from the switch in AO phase -Establish a climate record for the region north of Greenland through the retrieval and analysis of sediment cores -Improve an existing dynamic-thermodynamic sea ice model, focusing on the heavily deformed ice common in the region -Relate the region-specific changes which have occurred to the larger-scale Arctic variablity pattern -Place the recent ice and climate variability for this critical region into the context of long term climate record, as reconstructed from sediment cores

Although the most visible effect of fish cage aquaculture is the output of particulate organic waste, 80% of the total nutrient losses from fish farming are plant-available as potentially eutrophicating substances. This project will assess the ability of commercially important seaweeds, cultivated in the immediate vicinity of caged fish, to reduce the impact of such nutrient releases. The algae cultivated in high nutrient sites will be tested as a food source for humans and for cultivated shellfish, and a model of the distribution of dissolved contaminants from sea-cage fish farms will be developed to predict the impact of introducing algal cultivation at any site.

Distribution • What is the current distribution of coral colonies in the North Sea? • Where are coral colonies located on the structures? • Do any colonies show evidence of exposure to drill cuttings? Monitoring & Environmental Recording • What hydrodynamic regime and levels of suspended particulate material are coral colonies exposed to? • Does the coral skeleton retain an archive of any past contamination? • Does skeletal growth vary over time and does this correlate with any past contamination? • How variable is the rate of coral growth and does this correlate with any environmental variables? Environmental Sensitivity • What effect does increased sediment load have on coral behaviour and physiology? • What effect does exposure to discharges (e.g. cuttings and produced water) have on coral behaviour and physiology? • Are such exposures realistic in the field?

(a) To assemble and further develop an integrative methodology for in situ evaluation of the effects of turbidity and hypoxia on fish physiological and/or behavioural performance. (b) To determine experimentally the threshold values beyond which oxygen and turbidity levels are liable to alter fish physiological and/or behavioural performance. (c) To integrate the results obtained in a conceptual and predictive model. Main expected achievements: [1] establishment of a link between laboratory studies, studies in mesocosms and field studies, using the most advanced techniques for monitoring behaviour in various environmental conditions. [2] an understanding of the impact of water turbidity and oxygenation on three major components of the behavioural repertoire of fish: habitat selection, predator-prey interactions and schooling-aggregation. [3] Predictive ability for the effect of the environmental variables studied on ecologically relevant behaviour.

1. To undertake a review of procedures used in the regulation and monitoring of marine cage fish farms in Norway, Scotland and elsewhere to be used as the basis for creating an appropriate set of protocols, monitoring systems and techniques for the control of such farms in Mediterranean conditions 2. To carry out a field research programme to provide appropriate data on the environmental impact of marine cage fish farms in a range of conditions in the eastern Mediterranean. 3. To develop a predictive model to simulate the environmental response at Mediterranean sea cage farms to differing cage stocking levels and feeding regimes. This will be designed as a management tool for both the industry and regulatory authorities.

1. To generate high-resolution quantitative palaeoceanographic/palaeoclimatic data from NE Atlantic coastal/shelf sites for the last 2000 years using a multidisciplinary approach 2. To develop novel palaeoclimatic tools for shallow marine settings by (i) calibrating the proxy data against instrumental datasets, (ii) contributing to transfer function development, and (iii) then to extrapolate back beyond the timescale of the instrumental data using the palaeoclimate record 3. To investigate the link between late Holocene climate variability detected in the shelf/coastal regions of western Europe and the variability of the oceanic heat flux associated with the North Atlantic thermohaline circulation, and to compare such variability with existing high-resolution terrestrial proxies to help determine forcing mechanisms behind such climate change 4. To lay a foundation for the identification of hazards and resources linked with, or forced by, such climate change.

1. To use a combination of archival and contemporary data to develop and test hypotheses on the impact of climatic change on rocky intertidal animals and plants. 2. Forecast future community changes based on Met. Office Hadley centre models and UKCIP models. 3. Establish a low-cost fit-for-purpose network to enable regular updates of climatic impact projections. 4. Assess and report likely consequences of predicted changes on coastal ecosystems. To provide general contextual time-series data to support marine management and monitoring. 5. Evaluate use of intertidal indicator species as sustainability indices. Disseminate the results as widely as possible. 6. Provide a basis for the development of a pan-European monitoring network.

1. Establish a network to measure environmental change in marine waters by undertaking long-term research and monitoring 2. Maintain and enhance existing long-term research programmes 3. Restart important discontinued long-term research programmes 4. Develop a quality controlled database of long-term marine data series 5. Deliver and interpret long-term and broad scale contextual information to inform water quality monitoring 6. Demonstrate the benefits of preserving and networking long-term time series programmes

1. Observations of the physics of vertical and open boundary exchange in Regions of Restricted Exchanges (REEs), leading to improved parameterisation of these processes in research and simplified models. 2. Study of the phytoplankton and pelagic micro-heterotrophs responsible for production and decomposition of organic material, and of sedimentation, benthic processes and benthic-pelagic coupling, in RREs, with the results expressed as basin-scale parameters. 3. Construction of closed budgets and coupled physical-biological research models for nutrient (especially nitrogen) and organic carbon cycling in RREs, allowing tests of hypotheses about biogeochemistry, water quality and the balance of organisms. 4. Construction of simplified 'screening' models for the definition, assessment and prediction of eutrophication, involving collaboration with 'end-users', and the use of these models to analyse the costs and benefits of amelioration scenarios.

1. The improvement of the scientific basis for the management of fisheries taking elasmobranch species by: a)Species Identification /biological sampling b)Stock discrimination / separation c)Data compliation and exchange d)Data preparation, stock assessment & species vulnerability

1. To quantify the effectiveness of the biofilters in reducing the impacts of mariculture across Europe from both an economic and environmental perspective. 2. To determine the best design and placements of the biofilters, accounting for differences in geography, hydrology, nutrient input etc. between countries. 3. To examine the environmental and regulatory options governing the use of the biofilters at the end of their life-span and to provide detailed economic analyses of biofilter use compared to existing filtration methods.

1. To establish an environmental monitoring regieme during and following the period of reef complex construction using, where possible, the same static monitoring sites and transects established during the pre-deployment research, in addition to new stations 2. To develop and test models that will predict ecosystem changes caused by artifical habitat manipulation. The main model will examine whole ecosystem changes. Other models will examine hydrological profile alterations, habitat fractal dimensions and socio-economic cost benefit analysis.

1. To descirbe and compare the phylogenetic diversity and distribution of the total bacterial flora associated with G catenatum cysts and vegetative cells. 2. To culture and identify bacteria from G catenatum, and identify/characterise any bacteria capable of autonomous PST production in G. catenatum 3. To examine the effect of cyst surface sterilisation and re-introduction of bacteria on PST production in G catenatum 4. Survey bacteria for quorum sensing capability (cell signaling) and detect in situ quorum sesing in xenic G. catenatum cultures, relating to toxicity development. 5. Develop molecular markers of cross species quorum sensing, facilitating analysis of quorum sensing in uncultivated bacteria.

Objective 1: To map the structural and genetic variability, the framework-constructing potential, and the longevity of Deep Water Coral (DWC) ecosystems Objective 2: To assess hydrographic and other local physical forcing factors affecting Benthic Boundary Layer (BBL) sediment particle dynamics and POC supply in the vicinity of DWC ecosystems Objective 3: To describe the DWC ecosystem, its dynamics and functioning; investigate coral biology and behaviour and assess coral sensitivity to natural and anthropogenic stressors Objective 4: To assign a sensitivity code, identify the major conservation issues (and increase public awareness), and make recommendations for the sustainable use of the DWC ecosystem

The project aims to develop Molecular Imprinted Polymer (MIP)sensors into practical tools for the monitoring of a number of pollutants listed in the EU Water Framework Directive. (Further details in commercial confidence)

1. To develop a deep water observation system 2. Detailed design document, workplan and risk register and reviewed and agreed by steering group, procurement of components. 3. Deep water tests of acoustic communications system performed. pilot data dissemination and archival system. Dry test DWOS -1 4. Deployment in near lab test environment eg. Dunstaffnage bay with regular inspections. Collect, analyse, disseminate and archive sensor and house keeping data 5. Deploy in exposed but coastal stratified site in western Irish Sea, with two visual inspections. Collect, analyse, disseminate and archive sensor and house keeping data. Liaison with Met Office regarding deployment logistics. 6. Six months Deployment at Deep Water site; Collect, analyse, disseminate and archive sensor and house keeping data; Distribute data to customers. Revisit mooring site after six months recover and redeploy. 7. Final Technical Report and Final Project Report: Second six months Deployment at Deep Water site (as decreed by steering group); Collect, analyse, disseminate and archive sensor and house keeping data. Analysis of complete data handling chain performed; impact of data on customer base assessed, recommendations for continuance of DWOS as an operational system.

1. To determine the effects of each of several sealice treatment chemicals on macrofaunal assemblages 2. To determine the effects of each of several sealice treatment chemicals on zooplankton assemblages 3. To determine the effects of each of several sealice treatment chemicals on meiofaunal assemblages 4. To determine the effects of each of several sealice treatment chemicals on benthic diatom assemblages 5. To determine the effects of each of several sealice treatment chemicals on phytoplankton assemblages 6. To determine the effects of each of several sealice treatment chemicals on macroalgal and littoral assemblages 7. To measure the concentrations of each of several sea lice treatment chemicals in the environment post-treatment 8. To determine the significant correlations between ecosystem responses, time and therapeutant concentration to determine the proportion of the observed environmental variance attributal to the treatments against a background of responses due to other parameters such as waste organic materials and nutrients 9. To model the dispersion and or depostion of farm wastes including of each of several sea lice treatment chemicals in the marine environment post treatment and to incorporate terms relating to the toxicity of these chemicals to certain parts of the ecosystem (e.g. the macrofauna)