Dunstaffnage Marine Laboratory: projects/activities

-Development of methods to enhance the rate of toxin depuration ( detoxification), especially in shellfish species of high economic value and prolonged retention e.g., King Scallops -Understanding the reaction products and metabolic transformations of toxins in shellfish tissues. -Determine the relationship between algal population dynamics ( including free cell and encysted stages ) to seasonal and spatial patterns of toxicity in shellfish populations. -Assess the effects of harmful algae on the various stages in the life history of shellfish ( Larvae, Spat, Adults ). -Investigate sampling frequencies and protocols ( live shellfish sampling ).

Although the most visible effect of fish cage aquaculture is the output of particulate organic waste, 80% of the total nutrient losses from fish farming are plant-available as potentially eutrophicating substances. This project will assess the ability of commercially important seaweeds, cultivated in the immediate vicinity of caged fish, to reduce the impact of such nutrient releases. The algae cultivated in high nutrient sites will be tested as a food source for humans and for cultivated shellfish, and a model of the distribution of dissolved contaminants from sea-cage fish farms will be developed to predict the impact of introducing algal cultivation at any site.

Distribution • What is the current distribution of coral colonies in the North Sea? • Where are coral colonies located on the structures? • Do any colonies show evidence of exposure to drill cuttings? Monitoring & Environmental Recording • What hydrodynamic regime and levels of suspended particulate material are coral colonies exposed to? • Does the coral skeleton retain an archive of any past contamination? • Does skeletal growth vary over time and does this correlate with any past contamination? • How variable is the rate of coral growth and does this correlate with any environmental variables? Environmental Sensitivity • What effect does increased sediment load have on coral behaviour and physiology? • What effect does exposure to discharges (e.g. cuttings and produced water) have on coral behaviour and physiology? • Are such exposures realistic in the field?

1. To undertake a review of procedures used in the regulation and monitoring of marine cage fish farms in Norway, Scotland and elsewhere to be used as the basis for creating an appropriate set of protocols, monitoring systems and techniques for the control of such farms in Mediterranean conditions 2. To carry out a field research programme to provide appropriate data on the environmental impact of marine cage fish farms in a range of conditions in the eastern Mediterranean. 3. To develop a predictive model to simulate the environmental response at Mediterranean sea cage farms to differing cage stocking levels and feeding regimes. This will be designed as a management tool for both the industry and regulatory authorities.

1. Observations of the physics of vertical and open boundary exchange in Regions of Restricted Exchanges (REEs), leading to improved parameterisation of these processes in research and simplified models. 2. Study of the phytoplankton and pelagic micro-heterotrophs responsible for production and decomposition of organic material, and of sedimentation, benthic processes and benthic-pelagic coupling, in RREs, with the results expressed as basin-scale parameters. 3. Construction of closed budgets and coupled physical-biological research models for nutrient (especially nitrogen) and organic carbon cycling in RREs, allowing tests of hypotheses about biogeochemistry, water quality and the balance of organisms. 4. Construction of simplified 'screening' models for the definition, assessment and prediction of eutrophication, involving collaboration with 'end-users', and the use of these models to analyse the costs and benefits of amelioration scenarios.

1. To quantify the effectiveness of the biofilters in reducing the impacts of mariculture across Europe from both an economic and environmental perspective. 2. To determine the best design and placements of the biofilters, accounting for differences in geography, hydrology, nutrient input etc. between countries. 3. To examine the environmental and regulatory options governing the use of the biofilters at the end of their life-span and to provide detailed economic analyses of biofilter use compared to existing filtration methods.

1. Analysis of existing data from the current shellfish monitoring programmes in order to design a suitable sampling strategy 2. Ideentification of toxic algal species in UK waters 3. Construction of a detailed time-series at several key sites in the UK for toxic phytoplankton and shellfish toxin occurence 4. Comparison of the genotype versus toxicity of suspected toxic species between sites

The project aims to develop Molecular Imprinted Polymer (MIP)sensors into practical tools for the monitoring of a number of pollutants listed in the EU Water Framework Directive. (Further details in commercial confidence)

1. To determine the effects of each of several sealice treatment chemicals on macrofaunal assemblages 2. To determine the effects of each of several sealice treatment chemicals on zooplankton assemblages 3. To determine the effects of each of several sealice treatment chemicals on meiofaunal assemblages 4. To determine the effects of each of several sealice treatment chemicals on benthic diatom assemblages 5. To determine the effects of each of several sealice treatment chemicals on phytoplankton assemblages 6. To determine the effects of each of several sealice treatment chemicals on macroalgal and littoral assemblages 7. To measure the concentrations of each of several sea lice treatment chemicals in the environment post-treatment 8. To determine the significant correlations between ecosystem responses, time and therapeutant concentration to determine the proportion of the observed environmental variance attributal to the treatments against a background of responses due to other parameters such as waste organic materials and nutrients 9. To model the dispersion and or depostion of farm wastes including of each of several sea lice treatment chemicals in the marine environment post treatment and to incorporate terms relating to the toxicity of these chemicals to certain parts of the ecosystem (e.g. the macrofauna)