Dunstaffnage Marine Laboratory: projects/activities

Although the most visible effect of fish cage aquaculture is the output of particulate organic waste, 80% of the total nutrient losses from fish farming are plant-available as potentially eutrophicating substances. This project will assess the ability of commercially important seaweeds, cultivated in the immediate vicinity of caged fish, to reduce the impact of such nutrient releases. The algae cultivated in high nutrient sites will be tested as a food source for humans and for cultivated shellfish, and a model of the distribution of dissolved contaminants from sea-cage fish farms will be developed to predict the impact of introducing algal cultivation at any site.

Distribution • What is the current distribution of coral colonies in the North Sea? • Where are coral colonies located on the structures? • Do any colonies show evidence of exposure to drill cuttings? Monitoring & Environmental Recording • What hydrodynamic regime and levels of suspended particulate material are coral colonies exposed to? • Does the coral skeleton retain an archive of any past contamination? • Does skeletal growth vary over time and does this correlate with any past contamination? • How variable is the rate of coral growth and does this correlate with any environmental variables? Environmental Sensitivity • What effect does increased sediment load have on coral behaviour and physiology? • What effect does exposure to discharges (e.g. cuttings and produced water) have on coral behaviour and physiology? • Are such exposures realistic in the field?

(a) To assemble and further develop an integrative methodology for in situ evaluation of the effects of turbidity and hypoxia on fish physiological and/or behavioural performance. (b) To determine experimentally the threshold values beyond which oxygen and turbidity levels are liable to alter fish physiological and/or behavioural performance. (c) To integrate the results obtained in a conceptual and predictive model. Main expected achievements: [1] establishment of a link between laboratory studies, studies in mesocosms and field studies, using the most advanced techniques for monitoring behaviour in various environmental conditions. [2] an understanding of the impact of water turbidity and oxygenation on three major components of the behavioural repertoire of fish: habitat selection, predator-prey interactions and schooling-aggregation. [3] Predictive ability for the effect of the environmental variables studied on ecologically relevant behaviour.

1. To quantify benthic community parameters for all size classes of fauna across the Oxygen Minimum Zone (OMZ) 2. To make a detailed assessment across the OMZ of a) sediment accumulation, mixing and irrigation rates and depths and b) environmental factors acting as controls on faunal activity 3. To characterise solid phase and porewater geochemistry of sediments across the OMZ 4. To assess a) faunal digestive Organic Matter (OM) alteration, b) the relative importance of chemo- and photosynthetic food sources, and c) benthic food web structure, across the OMZ 5. To determine porewater profiles and benthic solute fluxes in situ, and to assess faunal OM assimilation and trophic relationships by monitoring tracers during shipboard and in situ incubations 6. To obtain high resolution porewater profiles of oxygen and other key analytes, free of pressure and other effects potentially introduced by core recovery 7. To determine in situ oxygen consumption rates and benthic fluxes 8. To use labelled tracers to assess mixing and irrigation rates, faunal OM assimilation, and size-selective ingestion and mixing 9.To determine sediment denitrification and sulfate reduction rates and their contributions to OM remineralisation

1. To undertake a review of procedures used in the regulation and monitoring of marine cage fish farms in Norway, Scotland and elsewhere to be used as the basis for creating an appropriate set of protocols, monitoring systems and techniques for the control of such farms in Mediterranean conditions 2. To carry out a field research programme to provide appropriate data on the environmental impact of marine cage fish farms in a range of conditions in the eastern Mediterranean. 3. To develop a predictive model to simulate the environmental response at Mediterranean sea cage farms to differing cage stocking levels and feeding regimes. This will be designed as a management tool for both the industry and regulatory authorities.

1. Establish a network to measure environmental change in marine waters by undertaking long-term research and monitoring 2. Maintain and enhance existing long-term research programmes 3. Restart important discontinued long-term research programmes 4. Develop a quality controlled database of long-term marine data series 5. Deliver and interpret long-term and broad scale contextual information to inform water quality monitoring 6. Demonstrate the benefits of preserving and networking long-term time series programmes

1. Observations of the physics of vertical and open boundary exchange in Regions of Restricted Exchanges (REEs), leading to improved parameterisation of these processes in research and simplified models. 2. Study of the phytoplankton and pelagic micro-heterotrophs responsible for production and decomposition of organic material, and of sedimentation, benthic processes and benthic-pelagic coupling, in RREs, with the results expressed as basin-scale parameters. 3. Construction of closed budgets and coupled physical-biological research models for nutrient (especially nitrogen) and organic carbon cycling in RREs, allowing tests of hypotheses about biogeochemistry, water quality and the balance of organisms. 4. Construction of simplified 'screening' models for the definition, assessment and prediction of eutrophication, involving collaboration with 'end-users', and the use of these models to analyse the costs and benefits of amelioration scenarios.

1. To quantify the effectiveness of the biofilters in reducing the impacts of mariculture across Europe from both an economic and environmental perspective. 2. To determine the best design and placements of the biofilters, accounting for differences in geography, hydrology, nutrient input etc. between countries. 3. To examine the environmental and regulatory options governing the use of the biofilters at the end of their life-span and to provide detailed economic analyses of biofilter use compared to existing filtration methods.

1. To descirbe and compare the phylogenetic diversity and distribution of the total bacterial flora associated with G catenatum cysts and vegetative cells. 2. To culture and identify bacteria from G catenatum, and identify/characterise any bacteria capable of autonomous PST production in G. catenatum 3. To examine the effect of cyst surface sterilisation and re-introduction of bacteria on PST production in G catenatum 4. Survey bacteria for quorum sensing capability (cell signaling) and detect in situ quorum sesing in xenic G. catenatum cultures, relating to toxicity development. 5. Develop molecular markers of cross species quorum sensing, facilitating analysis of quorum sensing in uncultivated bacteria.

1. Analysis of existing data from the current shellfish monitoring programmes in order to design a suitable sampling strategy 2. Ideentification of toxic algal species in UK waters 3. Construction of a detailed time-series at several key sites in the UK for toxic phytoplankton and shellfish toxin occurence 4. Comparison of the genotype versus toxicity of suspected toxic species between sites

1. To determine the effects of each of several sealice treatment chemicals on macrofaunal assemblages 2. To determine the effects of each of several sealice treatment chemicals on zooplankton assemblages 3. To determine the effects of each of several sealice treatment chemicals on meiofaunal assemblages 4. To determine the effects of each of several sealice treatment chemicals on benthic diatom assemblages 5. To determine the effects of each of several sealice treatment chemicals on phytoplankton assemblages 6. To determine the effects of each of several sealice treatment chemicals on macroalgal and littoral assemblages 7. To measure the concentrations of each of several sea lice treatment chemicals in the environment post-treatment 8. To determine the significant correlations between ecosystem responses, time and therapeutant concentration to determine the proportion of the observed environmental variance attributal to the treatments against a background of responses due to other parameters such as waste organic materials and nutrients 9. To model the dispersion and or depostion of farm wastes including of each of several sea lice treatment chemicals in the marine environment post treatment and to incorporate terms relating to the toxicity of these chemicals to certain parts of the ecosystem (e.g. the macrofauna)

1. To develop a system of photoactive biocides for treating sea lice and biofouling (Further details in confidence)