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White whale (Delphinapterus leucas) blubber samples from three of the five different Alaskan stocks - Cook Inlet (n = 20), Eastern Chukchi Sea (n = 19) and Eastern Beaufort Sea (n = 2) - were analyzed for levels and patterns of chemical contaminants. Blubber of these whales contained sum PCBs, sum DDTs, sum chlordanes, HCB, dieldrin, mirex, *toxaphene and *HCH, generally in concentration ranges similar to those found in white whales from the Canadian Arctic and lower than those in white whales from the highly contaminated St. Lawrence River. The males of the Cook Inlet and Eastern Chukchi Sea stocks had higher mean concentrations of all contaminant groups than did the females of the same stock, a result attributable to the transfer of these organochlorine contaminants (OCs) from the mother to the calf during pregnancy and during lactation following birth. Principal components analysis of patterns of contaminants present in blubber showed that Cook Inlet stock appeared to have identifiable contaminant patterns that allowed the stock to be distinguished from the others. Our results also showed that blubber from the three Alaskan stocks was a source of contaminant exposure for human subsistence consumers, but the health risks from consumption are currently unknown.
Blubber samples from Alaska ringed seal (Phoca hispida) were collected for inclusion in the US National Biomonitoring Specimen Bank, as well as for immediate analysis as part of the contaminant monitoring component of the US National Marine Fisheries Service's Marine Mammal Health and Stranding Response Program. The blubber samples were analyzed for organochlorine (OC) contaminants (e.g., PCB congeners, pesticides, DDTs). Results for ringed seals from the Alaska Arctic revealed low blubber concentrations of OC contaminants. Differences in contaminant concentrations among the Alaska seals may be explained by differences in feeding habits and migratory patterns; age or gender did not appear to account for the differences observed. The integration of real-time contaminant monitoring with specimen banking provides important baseline data that can be used to plan and manage banking activities. This includes identifying appropriate specimens that are useful in assessing temporal trends and increasing the utility of the banked samples in assessing chemical contaminant accumulation and relationships to biological effects.
1. Research area # 2 in the 1998/99 Announcement of Opportunity by CIFAR, "Study of anthropogenic influences on the Western Arctic/Bering Sea Ecosystem", and 2. Research area #4 in the 1998/99 Announcement of Opportunity by CIFAR, "Contaminant inputs, fate and effects on the ecosystem" specifically addressing objectives a-c, except "effects." a. "Determine pathways/linkages of contaminant accumulation in species that are consumed by top predators, including humans, and determine sub-regional differences in contaminant levels..." b. "Use an ecosystems approach to determine the effects of contaminants on food web and biomagnification." c. "Encourage local community participation in planning and implementing research strategies." The objectives of Phase I, Human Ecology Research are to: 1. Document reliance by indigenous arctic marine communities in Canada, Alaska and Russia on arctic resources at risk from chemical pollutants; and, 2. Incorporate traditional knowledge systems of subsistence harvesting. The human ecology components of the project were conducted within the frameworks of indigenous environmental knowledge and community participation. Using participatory mapping techniques, semi-structured interviews and the direct participation of community members in research design, data collection and implementation, research and data collection on the human ecology of indigenous arctic marine communities was undertaken in the communities of Holman, NWT (1998), Wainwright, Alaska (1999), and is underway in Novoe Chaplino, Russia. (2000).
The first part of the present study evaluated tissue concentrations of twelve essential and non-essential elements in four arctic marine mammal species important as subsistence resources to indigenous Alaskans. Species sampled included: bowhead whales, beluga whales, ringed seals, and polar bears. Concentrations of As, Cd, Co, Cu, Pb, Mg, Mn, Hg, Mo, Se, Ag, and Zn, were analyzed in liver, kidney, muscle, blubber, and epidermis (the latter in cetaceans only). Elements that were identified as having tissue concentrations, which in domesticated species would have been considered higher than normal and/or even toxic, were Cd, Hg, Ag, and Se. However, the concentrations of these elements were consistent with previous reports for arctic marine mammals. Remaining elements were at concentrations within normal ranges for domesticated species, although Cu was found frequently at concentrations that would be considered marginal or deficient in terrestrial domesticated animals. Across-species comparisons revealed that Cd was highest in kidney, followed by liver in all four species. Its concentrations were frequently correlated with Cu, Zn, Hg, and Se. Cadmium accumulated with age in bowhead and beluga whales, especially in liver and kidney. The relationships between Cd and Hg, and between Cd and Se were believed to be due to mutual accretion with age, although direct interactions could not be ruled out, especially with respect to Cd and Se. Associations between Cd and Cu, and Cd and Zn were potentially attributable to mutual binding with the inducible protein, metallothionein. This assumption was supported by the observation that Cd:Zn ratios in liver and kidney displayed a significant linear relationship to age and that this ratio either increased slightly (in kidney and liver of bowheads) or remained constant (in kidney and liver of belugas) with age. In general, Se was highest in liver and kidney of all four species, where it was frequently at concentrations that would have been deemed elevated or toxic for domesticated species, although within ranges previously reported for arctic marine mammals. Selenium increased with age indices, and was highly correlated with Hg, and often with Cd as well. Mercury also increased with age, and liver contained the highest tissue concentration in the cetacean and pinniped species. The pattern of Se accumulation in polar bears differed, with highest concentrations found in kidney, which suggested that this tissue may be the primary site for Hg detoxification in this species, as is the case for terrestrial mammals. Compared to the other three species, bowhead whales had very low Hg concentrations in all tissues. The highly significant linear relationship between Hg and Se noted in various tissues (particularly liver) of all four species was presumed due to binding of these two elements to each other following demethylation of MHg. This assumption was supported by the observations that while Se and Hg both accumulated with age, the fraction of total Hg that was composed of MHg decreased with age. The quantity that represented the difference between total Hg measured directly and calculated total Hg [i.e., SHg = Hg(II) + MHg], also increased with age in beluga liver. This connoted that a portion of the total Hg present was in an organic form other than MHg, and that this form accumulated with age. Alternatively, this portion, which was apparently not measured by either the Hg(II) or MHg procedures, may have been lost during extraction. Species in this study had mean hepatic Hg:Se molar ratios that were below unity. This implies that Hg concentrations may have been below some threshold level, after which subsequent accumulation proceeds in a 1:1 molar ratio fashion with Se. Alternatively, it might suggest that a 1:1 Hg:Se molar ratio is not a prerequisite for protection from Hg toxcosis among marine mammals, because none of the animals in the present study exhibited lesions typically associated with Hg toxicosis. In beluga liver, concentrations of Ag were elevated when compared to domesticated species. The only element that showed a significant linear association to Ag was Cu—a relationship that was observed in all four species. This suggested that Ag and Cu may be associated through a common ligand, possibly metallothionein. The association between Ag and Se in beluga liver was less strong than that between Hg and Se; moreover, Ag did not increase with age. These findings indicate that Ag probably does not compete with Hg for Se binding, and therefore is unlikely to substantially inhibit detoxification of Hg in beluga whales. In the second portion of this research, tissues from bowhead whales, beluga whales and ringed seals were examined at both the gross and light microscopic level. The purpose of this evaluation was three-fold: to describe the normal histologic appearance of tissues; to perform a routine histologic survey of tissues that would contribute to a general health assessment, and; to scrutinize tissues for lesions that might support a diagnosis of toxicosis caused by Cd, Hg, Ag, or Se. Tissues examined were chosen on the basis of their propensity to be targets for toxicologic injury from the specified elements (with the exception of brain) and included, but were not limited to, the tissues analyzed chemically. Special stains were used to identify particular pigments or tissue components. Overall, the bowhead whales evaluated appeared healthy and had low parasite burdens. The most common lesion, which was observed in all bowheads, was a non-inflammatory chronic renal periglomerular and interstitial fibrosis. This lesion was not typical of Cd-induced nephropathy, and it did not appear to be associated with renal Cd burdens. Nevertheless, thresholds of Cd-induced renal injury are not known for cetacean species, and more whales need to be examined histologically in conjunction with analysis of tissue Cd residues. Acute myodegeneration was observed in cardiac and/or skeletal muscle of a few bowheads, and was presumed to reflect a hunting-induced exertional myopathy. The beluga whales examined were generally in good body condition and appeared healthy grossly, but they had much higher parasite burdens than bowhead whales. In particular, prevalence in belugas of pulmonary nematodiasis was high, being especially common among whales obtained from Pt. Hope compared to those from Pt. Lay. Grossly, firm, caseous nodules were associated with lungworms, while histologically, the associated pulmonary changes ranged from mild chronic inflammation and focal granuloma formation to catarrhal granulomatous and eosinophilic verminous bronchopneumonia. Another change observed in some belugas and believed to be associated with lungworm infection, was multifocal pulmonary arterial medial hypertrophy and degeneration. Beluga whales harvested at Pt. Lay (summer) frequently showed evidence of hepatic and pancreatic atrophy, while whales taken at Pt. Hope (spring) did not. This was believed to result from anorexia during migration—a supposition corroborated by the lack of stomach contents among Pt. Lay whales. Another prominent histologic finding among belugas was hepatic telangectasia, which occurred with significantly greater frequency and severity in Pt. Hope belugas than in those from Pt. Lay. The etiology and significance of this lesion could be not be ascertained, although it was not believed to be associated with any of the elements analyzed in this study. Mild thickening of Bowman’s capsule was seen frequently in belugas. However, this lesion was not typical of Hg or Cd-induced nephropathies, and did not appear correlated with kidney concentrations of these metals. This lesion was believed to be a normal consequence of aging in belugas, although a metal etiology for it could not be excluded irrefutably. In general, ringed seals were in good body condition and appeared healthy on gross examination. Among seals evaluated histologically, the most common finding was a mild, chronic, focal or periportal hepatitis, with focal hepatocellular necrosis sometimes apparent. Although a metal etiology for this lesion could not be definitively ruled out, in the absence of other lesions that would support a diagnosis of metal toxicosis, an infectious etiology was considered more credible. Two out of sixteen seals had embryologic remnants (an epidermoid cyst and an ultimobranchial cyst)—lesions that are usually considered incidental. While no toxic (metal or otherwise) etiology could be ascertained for these lesions, the incidence of retained embryologic remnants seemed high. A number of xenobiotics are known to be endocrine-disruptors, and the potential for such an etiology among these seals should be examined further. Lipofuscin deposition was ubiquitous among all three species examined histologically. Lipofuscin was most prevalent in hepatocytes, but also commonly was observed in various other tissue and cell types, especially in cardiac and skeletal myocytes, and in uriniferous tubular epithelial cells. The third portion of this study employed autometallographic (AMG) development of light microscopic tissue sections to amplify and localize deposition of inorganic Hg in liver and kidney of beluga and bowhead whales. No staining occurred among bowhead tissues, confirming the extremely low concentration of Hg determined through chemical analyses. In beluga kidney sections, AMG granules were seen throughout the uriniferous tubular epithelium, showing that Hg deposits throughout the nephric tubule, and not solely in the proximal tubular epithelium. In liver tissue, AMG granules were deposited primarily in periportal regions among whales with lower hepatic Hg burdens. In addition to periportal deposition, AMG granules were observed in pericentral and mid-zonal regions in the belugas sampled that had higher liver Hg concentrations (generally older animals). Granules were densely concentrated in stellate macrophages, especially near portal triads. Granules also were distributed in hepatocellular cytoplasm, generally concentrated toward the bile cannalicular domain of the cell. Granules were discrete, potentially indicating that Hg was confined within lysosomes. These observations suggested that inorganic Hg deposits initially in periportal regions of young animals, with subsequent accumulation occurring pericentrally, and finally, midzonally as the whales age. Computer-assisted densitometric analysis was used for semi-quantitative evaluation of AMG staining intensities. These AMG staining intensities were well correlated with concentrations of Hg determined via chemical analysis. Areas with AMG-staining were identified and compared with location of lipofuscin in the same field, visualized with fluorescent microscopy. While AMG granules and lipofuscin deposits sometimes were co-localized, they more often were not. In addition, abundant lipofuscin deposition was seen in livers of younger belugas with little to no Hg-catalyzed AMG staining. Also, lipofuscin concentrated predominantly in pericentral regions. These observations suggested that in the healthy marine mammals of this study, marked hepatic lipofuscin deposition most often occurred independently of Hg accumulation. Consequently, hepatic lipofuscin is likely to be a poor indicator of Hg-induced damage in belugas. The abundant lipofuscin deposition in livers of marine mammals was interpreted as most likely denoting a heightened exposure to oxidative stress that is probably inherent to a marine mammalian existence. These oxidative stressors may include a diet high in polyunsaturated fatty acids (PUFAs), alternating hypoxia and abundant oxygenation, and periodic bouts of anorexia associated with migration.
We conducted gamma spectrometric analyses on more than 200 arctic marine mammal tissue samples. These samples were primarily provided by subsistence hunters from northern Alaska, with a smaller number of samples from the Resolute region in Canada. The majority of samples (>90% ) had detectable levels of the anthropogenic radionuclide 137Cs, with a mean level observed in all samples of 0.67 Bq kg-1 dry weight ±0.81 (SD). Converted to wet weight, the mean was 0.21 Bq kg-1 ±0.19 SD. The median activity observed was 0.45 Bq kg-1 dry weight (0.18 Bq kg-1 wet weight) with a range from detection limits to 6.7 Bq kg-1dry weight (1.1 Bq kg-l wet weight). These findings confirm expectations that current anthropogenic gamma emitter burdens in marine mammals used in the North American Arctic as subsistence food resources are well below activities that would normally merit public health concern (~1000 Bq kg-1 wet weight). Some differences among species and tissues were observed. Beluga tissues had slightly higher mean burdens of 137Cs overall, and epidermis and muscle tissues in bowhead and beluga whales typically had higher burdens than other tissues analyzed. Low levels of the neutron activation product l08mAg (half-life 418 yr.), probably bioaccumulated from bomb fallout sources, were observed in 16 of 17 beluga livers analyzed, but were not found in any other tissues of beluga or in any other species sampled. A subset of 39 samples of various tissues was analyzed for the alpha and beta emitters 239,240Pu and 90Sr. Plutonium levels were near the threshold of detectability (~0.1 Bq kg-1 dry weight) in 6 of the 39 samples; all other samples had no detectable plutonium. A detectable level of 90Sr (10.3 ±1.0 Bq kg-1 dry weight) was observed in only one of the 39 samples analyzed, a bowhead epidermis sample. Although the accumulation of 108mAg has not been previously reported in any marine mammal livers, all of our analytical measurements indicate that only very low levels of anthropogenic radioactivity are associated with marine mammals harvested and consumed in the North American Arctic.