The P450 enzyme system of the Arctic charr as a biomarker of POP contamination in Arctic aquatic environments

Updated 2000-10-05

Validate the hepatic P450 enzyme system as a biomarker of levels and effects of POPs in Arctic, aquatic environments, using the anadromous (sea-migratory) Arctic charr as an indicator species.

This is not a National Implementation Plan (NIP) project

Time frame

Project time span
1995 -
Data collection
1995 -
Data processing
1995 -
Data reporting
1995 -

Contact information

Contact person
Even H. Jørgensen
Norwegain Institute for Nature Research Polar Environmental Centre N-9296 Tromsø Norway
+47 77 75 04 13
+47 77 75 04 01
Other project contacts
Hans Wolkers, Akvaplan niva, Polar Environmental Centre, N-9296 Tromsø, Norway Anders Goksøyr, Dept. of Molecular Biology, Univ. of Bergen, HIB, N-5020 Bergen, Norway Malin Celander, Gøteborg University, Dept. of Zoology, Box. 463, SE 405 30, Gøteborg, Sweden

Parameters and Media

Parameter groups measured/observed/modelled
Biological effects
Persistent organic pollutants (POPs)
Petroleum hydrocarbons
Media sampled/studied/modelled
Freshwater fish
Additional information or further specification of types of data / information collected, species / tissues / organs sampled, etc.

The project are mainly conducted with laboratory fish which are experimentally treated with organoclorines and polyaromatic hydrocarbones


Regions studied
Petroleum hydrocarbons
Stations or areas where observations are made

The anadromous Arctic charr are used as a model species, due to the fact that this species are circumpolary distributed and as such an ideal indicator species for the Arctic.

Data availability

Are data archived or planned to be archived at an AMAP Thematic Data Centre?
References to key publications (or planned publications) and data reports
Wolkers, J., Jørgensen, E.H., Nijmeier, S. M. and Witgkamp, R. F. 1996. Time dependent induction of two distinct hepatic cytochrome P4501A catalytic activities in Arctic charr (Salvelinus alpinus) after oral exposure to benzo(a)pyrene. Aquatic Toxicology. 35: 127-138. Wolkers, J., Jørgensen, E.H., Nijmeier, S. M. and Witgkamp, R. F. 1998. Dose and time dependency of cytochrome P4501A induction in liver and kidney of B(a)P exposed Arctic charr (Salvelinus alpinus). Mar. Environm. Res. 46: 117-120. Jørgensen, E.H., Bye, B.E. and Jobling, M. 1999. Influence of nutritional status on biomarker responses to PCB in the Arctic charr (Salvelinus alpinus). Aquatic Toxicology. 44: 233-244. Jørgensen, E.H. and Wolkers, J. 1999. The effect of temperature on the time-dependent P4501A response after oral exposure to benzo(a)pyrene in winter- and summer-acclimated Arctic charr (Salvelinus alpinus). Can. J. Fish. Aquat. Sci. 56: 1370-1375.
Samples/specimens archived in specimen banks?

Methods & Procedures

Procedures and methodology used for, e.g., sampling and sample storage, sample pretreatment, extraction and analysis, including which laboratories are involved, references to methods employed, etc.

Hepatic CYP1A enzyme activity and content are analysed according to methods described in Jørgensen and Wolkers, 1999 (Can. J. Fish. Aquat. Sci., 56, 1370-1375.

Additional Information

Is this a bi- AND multi-lateral project (i.e. a project involving cooperation between different countries)?
Other institutes involved in the project

Gøteborg University, Dept. of Zoology, Box. 463, SE 405 30, Gøteborg, Sweden

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