Marine foodwebs as vector and possibly source of viruses and bacteria patogenic to humans shall be investigated in a compartive north-south study. Effects of sewage from ships traffic and urban settlements, on animals of arctic foodwebs will be studied.
Water samples: virus concentrates, indicative sewage bact. Fish and shellfish: gut contents, hepatopancreas/liver. Birds: fresh guano, occ. exudates of diseased individuals. Mammals: fresh fecal material, occ. exudates and blood, exudates of diseased individuals. (Dead individuals of birds/mammals are not collected)
RNA and DNA extracts, stored at -80 degr.C. till 2007 at least, probably at Kristineberg Marine Station, Sweden. Second contact person: dr. Bodil Hernroth.
Analyses of DNA and RNA from pathogens are performed at the Virology lab. of GU, partly at KMF. Procedures are aseptic from sampling through all steps, and carried out according to published and recommended methods and good laboratory practise. Some samples are preserved on site, all are kept frozen until analysis and stored at -80 degr.C.
Both laboratories have implemented QA/QC systems, both at the procedure and management levels. Identification of pathogens is based upon specific genetic probe or standard, or when necessary, genetic sequencing data of PCR product.
Dept. of Microbiology and Virology, Sahlgenska Hospital, Göteborg University, Sweden prof. Tomas Bergström.
Financing for 2005 is applied at NFR, Oslo. No other reports are made so far.