Updated 2000-10-23
To clarify whether metals and/or POPs affect marine fish species - Atlantic cod (Gadus morhua) and
plaice (Pleuronectes platessa)
This is not a National Implementation Plan (NIP) project
Time frame
- Status
- Ongoing
- Project time span
- 2000 - 2002
- Data collection
- 2000 - 2002
- Data processing
- 2000 - 2002
- Data reporting
- 2001 - 2002
Geography
- Regions studied
- Marine TDC
- Marine TDC
- Stations or areas where observations are made
JAMP-stations 11 (Kirkenes) and 98 (Svolvær)
Data availability
- Are data archived or planned to be archived at an AMAP Thematic Data Centre?
- yes-partly
- Data centres
- Marine TDC
If no (or only part of data are reported to a TDC), where and how are (other) data stored?
The data will be made available to AMAP at the completion of each year's National Comments to OSPAR
- Samples/specimens archived in specimen banks?
- Yes
- Specimen banking information
Processed samples stored at NIVA
Methods & Procedures
- Procedures and methodology used for, e.g., sampling and sample storage, sample pretreatment, extraction and analysis, including which laboratories are involved, references to methods employed, etc.
(1) tissue sampled from fish and stored/transported in liguid nitrogen
(2) liver sample homogenised in buffer and cytosolic fractions separated
(3) metallothionein analysed in cytosolic fraction, cytochrome P4501A in microsomal fraction
(4) protein determined in both fraction and in blood cells (see below) by spectrophotometric method
(5) PAH metabolites analysed in bile by HPLC with a flourometric detector
(6) ALA-D activity in red blood cells analysed
- QA/QC Information (what QA/QC procedures are implemented, laboratories involvment in QA/QC activities, model verification/validation routines, etc.)
NIVA is a partner in the BEQUALM programme (EU-funded) and lead country for metallothionein and ALA-D analyses. We organise international intercalibration programmes for MT and ALA-D. We participate in programmes for EROD (cytochrome P4501A activity) and PAH metabolites in bile.
Additional Information
- Is this a bi- AND multi-lateral project (i.e. a project involving cooperation between different countries)?
- No
- Is this project reporting to other organizations/programmes?
OSPAR, data stored in ICES database
Part of the Norwegian JAMP