Effects of metals and POPs on marine fish species

Updated 2000-10-23

To clarify whether metals and/or POPs affect marine fish species - Atlantic cod (Gadus morhua) and plaice (Pleuronectes platessa)

This is not a National Implementation Plan (NIP) project

Comments and additional information:

Part of the Norwegian JAMP

Contact information

Parameter groups measured/observed/modelled

Media sampled/studied/modelled

Additional information or further specification of types of data / information collected, species / tissues / organs sampled, etc.

liver, blood and bile

Regions studied: Marine TDC; Marine TDC
Stations or areas where observations are made: JAMP-stations 11 (Kirkenes) and 98 (Svolvær)

Are data archived or planned to be archived at an AMAP Thematic Data Centre?: yes-partly

If no (or only part of data are reported to a TDC), where and how are (other) data stored?

The data will be made available to AMAP at the completion of each year's National Comments to OSPAR

Procedures and methodology used for, e.g., sampling and sample storage, sample pretreatment, extraction and analysis, including which laboratories are involved, references to methods employed, etc.: (1) tissue sampled from fish and stored/transported in liguid nitrogen (2) liver sample homogenised in buffer and cytosolic fractions separated (3) metallothionein analysed in cytosolic fraction, cytochrome P4501A in microsomal fraction (4) protein determined in both fraction and in blood cells (see below) by spectrophotometric method (5) PAH metabolites analysed in bile by HPLC with a flourometric detector (6) ALA-D activity in red blood cells analysed
QA/QC Information (what QA/QC procedures are implemented, laboratories involvment in QA/QC activities, model verification/validation routines, etc.): NIVA is a partner in the BEQUALM programme (EU-funded) and lead country for metallothionein and ALA-D analyses. We organise international intercalibration programmes for MT and ALA-D. We participate in programmes for EROD (cytochrome P4501A activity) and PAH metabolites in bile.

Is this a bi- AND multi-lateral project (i.e. a project involving cooperation between different countries)?: No
Is this project reporting to other organizations/programmes?: OSPAR, data stored in ICES database