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As a result of the increasing atmospheric CO2 levels and other greenhose gases due to anthropogenic activities, global and water temperature is rising. The objectives of our project might be summarized as follows: I. To measure the activity of the enzymatic systems involved in carbon, nitrogen and phosphorus uptake (carbonic anhydrase, nitrate reductase and alkaline phosphatase) in selected macroalgae. To assess the optimal concentration of inorganic nitrogen and phosphorus for growth and photosynthesis. To study the total concentration of carbon and nitrogen metabolites in the macroalgae (proteins, total carbohydrates, and lipids) in order to define the possible existence of nutrient limitation. II. To simulate the conditions of climate change, represented as CO2 enrichment and increasing UV radiation, on the activity of carbon, nitrogen and phosphorus uptake mechanisms. III. To screen the activity of the enzymatic systems previously detailed in macroalgae from the Konjsfjord, in order to know their nutritional state.
The overall objective is to assess the influence of increased UV radiation and temperature on photosynthesis, nutrient uptake and primary production of microphytes. In order to do this, the existence and nature of strategies against potential UV damage in marine macrophytes of different climatic regions will be investigated. Research activities Measurement of photosynthesis using oxygen exchange and variable fluorescence (PAM); determination of oxidative stress (Gluthation, SOD, CLSM) and nutrient uptake under different UV-regimes