The influence of body lipid status on PCB toxicokinetics in the anadromous Arctic charr (Salvelinus alpinus)

Updated 2000-10-05

The objective is to study the relationship between natural seasonal variations in body lipid status of sea migrating Arctic charr and disposition (e.g. tissue distribution)of PCB, particularly in relation to the toxical potential of a certain body burden of PCB.

This is not a National Implementation Plan (NIP) project
Comments and additional information:

The study is performed as an experimental contamination of wild, anadromous charr that are brought into the laboratory.

Time frame

Status
Ongoing
Project time span
1996 -
Data collection
1996 - 2002
Data processing
1996 -
Data reporting
1996 -

Contact information

Contact person
Even H. Jørgensen
Address
Norwegain Institute for Nature Research Polar Environmental Centre N-9296 Tromsø Norway
Phone
+47 77 75 04 13
Fax
+47 77 75 04 01
Email
on.ukinanin.sotanin@nesnegroj.neve
Other project contacts
Ivan C. Burkow Norwegain Institute of Air Research Polar Environmental Centre N-9296 Tromsø

Parameters and Media

Parameter groups measured/observed/modelled
Biological effects
Persistent organic pollutants (POPs)
Media sampled/studied/modelled
Freshwater fish
Marine fish
Additional information or further specification of types of data / information collected, species / tissues / organs sampled, etc.

The proportional distribution of the total body burden of PCB between tissues containing most of the stored lipids (e.g. muscle)and vital organs such as liver, kidney and brain are quantified. Furtehrmore, the biological response to PCB in realtion to tissue distribution is studied by quantifying the activity of the hepatic P450 1A enzymes.

Geography

Regions studied
Persistent organic pollutants (POPs)

Data availability

Are data archived or planned to be archived at an AMAP Thematic Data Centre?
no
References to key publications (or planned publications) and data reports
Jørgensen, E.H, Johansen, S.J.S. and Jobling, M. 1997. Seasonal pattern of growth, lipid deposition and lipid depletion in anadromous Arctic charr. J. Fish Biol. 51: 312-326. Jørgensen, E. H., Burkow, I. C., Foshaug, H., Killie, B. and Ingebrigtsen, K. 1997. The influence of lipid status on tissue distribution of the persistent organic pollutant octachlorostyrene in Arctic charr (Salvelinus alpinus). Comp. Biochem. Physiol. 118C: 311-318. Kallenborn, R., Burkow, I.C., Schlabach, M. and Jørgensen, E.H. 1997. PCB and pesticide distribution in cod (Gadus morhua), sea trout (Salmo trutta) and Arctic charr (Salvelinus alpinus) from the Norwegian Arctic. Organohalogen Compounds 32: 252-256. Jobling, M., Johansen, J.S., Foshaug, H., Burkow, I.C. and Jørgensen, E.H. 1998. Lipid dynamics in anadromous Arctic charr, Salvelinus alpinus (L.): seasonal variations in lipid storage depots and lipid class composition. Fish Physiol. Biochem. 18: 225-240. Jørgensen, E.H., Bye, B.E. and Jobling, M. 1999. Influence of nutritional status on biomarker responses to PCB in the Arctic charr (Salvelinus alpinus). Aquatic Toxicology. 44: 233-244. Jørgensen, E.H. and Burkow, I.C. 1997. A model for investigating the fate and sub-lethal effects of lipophilic organochlorines in Arctic fish. Proceeding, AMAP International Symposium on Environmental Pollution of the Arctic. Tromsø, Norway, pp. 1-5. Foshaug, H., Jørgensen, E. H., Plotitsyna, N. and Burkow, I.C. 2000. Disposition of PCB during the winter emaciation of the anadromous Arctic charr. Proceeding, International Congress on the Biology of Fish, Aberdeen, UK. pp 55-65.
Samples/specimens archived in specimen banks?
No

Methods & Procedures

Procedures and methodology used for, e.g., sampling and sample storage, sample pretreatment, extraction and analysis, including which laboratories are involved, references to methods employed, etc.

Fish are contaminated by gastric implantation. Total lipids and lipid composition are analysed by extraction and high-performance thin-layer chromatography. PCB are analysed by cyclohexane extraction and capillary gas chromatography. All analyses are performed at the NILU laboratories either at Kjeller or in Tromsø. EROD activity are quantified by standard methods (Wolkers et al., 1996. Aquat. toxicol., 35: 127-138).

QA/QC Information (what QA/QC procedures are implemented, laboratories involvment in QA/QC activities, model verification/validation routines, etc.)

PCB analyses in accordence with EU-standard EN 45001.

Additional Information

Is this a bi- AND multi-lateral project (i.e. a project involving cooperation between different countries)?
Yes
Other institutes involved in the project

Miljøkjemisk avdeling, Universitetet i Umeå, Sverige

Is this project reporting to other organizations/programmes?

One PhD student, Hanne Foshaug, are founded by the Norwegian Research Council at this project.

Other related projects

1) Xenobiotic impact on Arctic charr: Nutritional modulation and physiological consequences 2) The P450 enzymesystem of the Arctic charr as a biomarker of POP contamination in the Arctic aquatic environment.

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