Persistent organic pollutants measurements in Arctic fox from the Pribilof Islands, Alaska

Updated 2001-03-16

Objectives were to measure a suite of organochlorine contaminants in tissues of Arctic fox collected on the Pribilof Islands for comparison to similar measurements in Arctic fox from other locations for the AMAP assessment.

This is not a National Implementation Plan (NIP) project
Comments and additional information:

Additional samples may be available in the summer of 2001 and will be analyzed when received.

Time frame

Status
Completed
Project time span
2000 - 2000
Data collection
1997 - 2000
Data processing
2000 - 2000
Data reporting
2000 - 2001

Contact information

Contact person
Margaret M. Krahn
Address
Environmental Conservation Division Northwest Fisheries Science Center National Marine Fisheries Service National Oceanic and Atmospheric Administration 2725 Montlake Boulevard East Seattle, WA 98112 USA.
Phone
+1 206 860 3326
Fax
+1 206 860 3335
Email
vog.aaon@nharK.yggeP
Other project contacts
Paula White Museum of Vertebrate Zoology University of California Berkeley 3101 Valley Life Sciences Building Berkeley, CA 94720 (510) 642-7888 pawhite@socrates.berkeley.edu Gina Ylitalo Environmental Conservation Division Northwest Fisheries Science Center National Marine Fisheries Service National Oceanic and Atmospheric Administration 2725 Montlake Boulevard East Seattle, WA 98112 USA. gina.ylitalo@noaa.gov Donald Brown Environmental Conservation Division Northwest Fisheries Science Center National Marine Fisheries Service National Oceanic and Atmospheric Administration 2725 Montlake Boulevard East Seattle, WA 98112 USA. donald.w.brown@noaa.gov

Parameters and Media

Parameter groups measured/observed/modelled
Persistent organic pollutants (POPs)
Media sampled/studied/modelled
Terrestrial mammals
Additional information or further specification of types of data / information collected, species / tissues / organs sampled, etc.

Body fat samples taken from the carcasses of Arctic foxes found dead or dying. Arctic fox (length, weight, age estimate, sex, fat sample)

Geography

Regions studied
Alaska
Bering Sea
Other areas
Pribilof Islands
Stations or areas where observations are made

St. Paul Island, Pribilof Islands (57.1667° N, 170.2500° W) Arctic fox (length, weight, age estimate, sex, fat sample)

Data availability

Are data archived or planned to be archived at an AMAP Thematic Data Centre?
yes-all
Data centres
Marine TDC
Samples/specimens archived in specimen banks?
No

Methods & Procedures

Procedures and methodology used for, e.g., sampling and sample storage, sample pretreatment, extraction and analysis, including which laboratories are involved, references to methods employed, etc.

Samples were stored frozen (-20 degrees C) until subsampled. Samples of fat were removed from the rump with cleaned scalpel blades and transferred to clean glass containers and returned to the freezer until analyis. Fat samples were analyzed for OCs and percent lipid following standard methods and quality assurance protocols (Krahn et al., 1988; Sloan et al., 1993). Briefly, samples of thawed tissue (1-3g ) were extracted, following addition of internal standards, by maceration with sodium sulfate and methylene chloride. The methylene chloride extract was then filtered through a column of silica gel and alumina and concentrated for further cleanup. High-performance liquid chromatography (HPLC) with a size-exclusion column was used to separate lipids and other biogenic material from a fraction containing the OCs. The OC fraction was analyzed by capillary column gas chromatography (GC) equipped with an electron capture detector (ECD). Identification of individual OCs in selected samples was confirmed using GC-mass spectrometry. References: Krahn, M.M., Moore, L.K., Bogar, R.G., Wigren, C.A., Chan, S.-L. and Brown, D.W. 1988. High-performance liquid chromatographic method for isolating organic contaminants from tissue and sediment extracts. J. Chromatogr. 437:161-75. Sloan, C.A., Adams, N.G., Pearce, R.W., Brown, D.W. and Chan, S.-L. 1993. Northwest Fisheries Science Center Organic Analytical Procedures. pp. 53-96. In: (G.G. Lauenstein and A.Y. Cantillo, ed.) Sampling and analytical methods of the National Status and Trends Program: National Benthic Surveillance and Mussel Watch Projects 1984-1992. NOAA Coastal Monitoring and Bioeffects Assessment Division, Office of Ocean Resources Conservation and Assessment, National Ocean Service, Silver Spring, Maryland, 182pp.

QA/QC Information (what QA/QC procedures are implemented, laboratories involvment in QA/QC activities, model verification/validation routines, etc.)

Quality assurance procedures included analyses of National Institute of Standards and Technology (NIST) standard reference materials (NIST SRM 1945), a NIST whale blubber control material, certified calibration standards, method blanks, solvent blanks and replicate samples and met laboratory criteria. In addition, our laboratory participates in NIST and other Quality Assurance Intercomparison Exercises each year.

Additional Information

Is this a bi- AND multi-lateral project (i.e. a project involving cooperation between different countries)?
No
Other institutes involved in the project

Paula White, University of California Berkeley.

Is this project reporting to other organizations/programmes?

This work has been done in collaboration with Paula White, University of California Berkeley.

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