Metal effects in Arctic seals

Updated 2000-10-23

To clarify whether effects of metals (Cd, Hg) affects biochemical markers (MT) in seal kidneys

This is not a National Implementation Plan (NIP) project

Time frame

Status
Ongoing
Project time span
2000 - 2002
Data collection
2000 - 2002
Data processing
2000 - 2002
Data reporting
2001 - 2002

Contact information

Contact person
Ketil Hylland
Address
NIVA P.O.Box 173, Kjelsås N-0411 Oslo Norway
Phone
+47 22 18 51 70
Fax
+47 22 18 52 00
Email
on.avin@dnallyh.litek
Other project contacts
DMU-Denmark (Rune Dietz)

Parameters and Media

Parameter groups measured/observed/modelled
Biological effects
Heavy metals
Media sampled/studied/modelled
Marine mammals
Additional information or further specification of types of data / information collected, species / tissues / organs sampled, etc.

kidney tissues

Geography

Regions studied
Greenland

Data availability

Are data archived or planned to be archived at an AMAP Thematic Data Centre?
yes-partly
Data centres
Marine TDC
Samples/specimens archived in specimen banks?
Yes
Specimen banking information

Processed samples stored at NIVA

Methods & Procedures

Procedures and methodology used for, e.g., sampling and sample storage, sample pretreatment, extraction and analysis, including which laboratories are involved, references to methods employed, etc.

(1) tissue sampled from seals and stored/transported in liguid nitrogen (2) sample homogenised in buffer and cytosolic fractions separated (3) metallothionein analysed in cytosolic fraction by differential pulse polarography and HPLC (4) protein determined in cytosolic fraction by spectrophotometric method

QA/QC Information (what QA/QC procedures are implemented, laboratories involvment in QA/QC activities, model verification/validation routines, etc.)

NIVA is a partner in the BEQUALM programme (EU-funded) and lead country for metallothionein analyses. Mammalian metallothionein is not included in the programme at present, so internal QA procedures will be used. The protocol includes the use of reference materials.

Additional Information

Is this a bi- AND multi-lateral project (i.e. a project involving cooperation between different countries)?
Yes
Other institutes involved in the project

DMU (DK), HI (NO)

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